Real-time PCR probe optimization using design of experiments approach
نویسندگان
چکیده
منابع مشابه
Real-time PCR probe optimization using design of experiments approach
Primer and probe sequence designs are among the most critical input factors in real-time polymerase chain reaction (PCR) assay optimization. In this study, we present the use of statistical design of experiments (DOE) approach as a general guideline for probe optimization and more specifically focus on design optimization of label-free hydrolysis probes that are designated as mediator probes (M...
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ژورنال
عنوان ژورنال: Biomolecular Detection and Quantification
سال: 2016
ISSN: 2214-7535
DOI: 10.1016/j.bdq.2015.12.002